Table 1.

Primers.

Guide RNA Sequences
p53 sgRNA	CCTCGAGCTCCCTCTGAGCC
Pten sgRNA	GAGATCGTTAGCAGAAACAAA
Cr8 sgRNA	GACATTTCTTTCCCCACTGG
Primers used in T7 Endonuclease Mutation Detection Assay
T7 Mut PCR p53 fwd	GCCATCTTGGGTCCTGACTT
T7 Mut PCR p53 rev	CCCCGCAGGATTTACAGACA
T7 Mut PCR Pten fwd	GAGCCATTTCCATCCTGCAG
T7 Mut PCR Pten rev	CTAGCCGAACACTCCCTAGG
T7 Mut PCR Cr8 fwd	TAAGATGATTATCTGAATTCCTGGG
T7 Mut PCR Cr8 rev	TCTTATCCCCTGTGTTGGAA
Primers Used in NGS
NGS PCR p53 fwd	CCATAGGGGTTTGTTTGTTTGT
NGS PCR p53 rev	CGCAGGATTTACAGACACCC
NGS PCR Pten fwd	GAGCCATTTCCATCCTGCAG
NGS PCR Pten rev	CACGATCTAGAAATGCGCCC
NGS PCR Cr8 fwd	TCTGAATTCCTGGGATGGGG
NGS PCR Cr8 rev	TGTGTGGCTACCCTGTTCTT