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. 2019 Nov 22;11(12):1848. doi: 10.3390/cancers11121848

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Paclitaxel-induced apoptosis of Detroit 562, FaDu and SCC25 cells. Annexin V-FLUOS/ propidium iodide (Ann/PI)-stained HNSCC cells were analyzed by FACS (fluorescence-activated cell sorting) after 48 h of treatment with paclitaxel different concentrations. Live cells are presented by the Ann-/PI- fraction, apoptotic cells by the Ann+/PI- fraction, secondary necrotic cells by the Ann+/PI+ fraction and primary necrotic cells are detected in the Ann-/PI+ fraction. Data are presented as mean ± SD. Statistical analysis was performed by Student’s t-test, the cell fractions at all concentrations were compared to control fractions in each cell line * p < 0.05.