Table 5.
Antibodies and corresponding protocols used (antigen retrieval, concentration).
| Provider | Antigen Retrieval | Automate | Dilution | |
|---|---|---|---|---|
| CD3 | Roche | pH9 | Ventana, Roche | RTU |
| CD79a | Roche | pH9 | Ventana, Roche | RTU |
| CD15 | Roche | pH9 | Ventana, Roche | RTU |
| CD30 | Roche | pH9 | Ventana, Roche | RTU |
| CD45 | Roche | pH9 | Ventana, Roche | RTU |
| CD68-PGM1 | Clinisciences | No | Ventana, Roche | 1/100 |
| CD56 | Roche | pH9 | Ventana, Roche | RTU |
| AE1/AE3 | Agilent | No | Autostainer, Dako | RTU |
| p63 | Agilent | pH9 | Autostainer, Dako | 1/50 |
| p40 | Dako | pH6 | Autostainer, Dako | 1/200 |
| CK5/6 | Agilent | No | Autostainer, Dako | 1/100 |
| CK7 | Agilent | No | Autostainer, Dako | 1/400 |
| CK20 | Agilent | No | Autostainer, Dako | 1/200 |
| TTF1 | Agilent | pH6 | Autostainer, Dako | RTU |
| Chromogranin | Agilent | No | Autostainer, Dako | 1/100 |
| Synpatophysin | MM France | pH9 | Autostainer, Dako | RTU |
| Ki67 | Agilent | pH6 | Autostainer, Dako | 1/50 |
| Mammaglobin | Dako | No | Autostainer, Dako | RTU |
| Desmin | Agilent | No | Autostainer, Dako | 1/100 |
| Vimentin | Dako | No | Autostainer, Dako | RTU |
| Smooth Muscle Actin | Agilent | No | Autostainer, Dako | RTU |
| Androgen Receptor | Dako | pH9 | Autostainer, Dako | 1/200 |
| MelanA | Agilent | No | Autostainer, Dako | RTU |
| (1) |
RTU: ready-to-use.