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. 2019 Dec 13;11(12):2011. doi: 10.3390/cancers11122011

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Protein expression of DAGLA and CEACMA1 in CAP-treated MCF-7/TxR cells. The protein expression of DAGLA (A) and CEACAM1 (B) in CAP-exposed MCF-7/TxR cells was examined by Western blot analysis. The protein expression of DAGLA and CEACAM1 showed a similar profile to that of microarray and qPCR. (C,D) The effect of CAP on the expression of DAGLA and CEACAM1 was examined after NAC treatment. NAC deteriorated the effect of CAP on the two proteins. The protein band in the Western blot was measured by a gel document system, and the levels are represented by bar graphs. All assays were performed in triplicate, and the results are depicted as mean ± SE. * p < 0.05, ** p < 0.01, *** p < 0.001.