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. 2019 Nov 11;6(1):40–54. doi: 10.1002/cjp2.145

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© 2019 The Authors. The Journal of Pathology: Clinical Research published by The Pathological Society of Great Britain and Ireland and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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H&E staining and histomorphology. Histomorphology of PFPE and FFPE H&E stained sections of tumour block (A), Temno biopsy (B) and tumour FNA clot preparations (C). Nuclear staining of Temno biopsies and tumour FNA specimens was preferable in PFPE samples (both p < 0.05). Scores for nuclear, cytoplasmic and cell membranous staining were combined for tumour blocks (D), Temno biopsies (E) and for tumour FNA samples (F) and correlations between FFPE and PFPE scores were observed for Temno biopsies (r ² = 0.3455, p = 0.0269) and tumour FNA samples (r ² = 0.8919, p < 0.0001). Representative H&E staining of PFPE adenocarcinoma (G) and PFPE squamous cell carcinoma (H) and FFPE adenocarcinoma (I) and FFPE squamous cell carcinoma (J).