Table 1.
Sequences of primers used for quantitative PCR
| Primer name (mouse) | Primer sequence 5′–3′ | Size (bp)a | Tm (°C) | GC (%) | Amplicon size (bp)a |
|---|---|---|---|---|---|
| Merg1a forward | cctcgacaccatcatccgca | 20 | 59.6 | 55.0 | 145 |
| Merg1a reverse | aggaaatcgcaggtgcaggg | 20 | 60.3 | 60.0 | |
| 18S subunit forward | cgccgctagaggtgaaattct | 21 | 57.2 | 52.4 | 101 |
| 18S subunit reverse | agaacgaaagtcggaggttc | 20 | 57.0 | 52.4 | |
| Calpain 1 forward | gctaccgtttgtctagcgtc | 20 | 58.73 | 55.0 | 98 |
| Calpain 1 reverse | taactcctctgtcatcctctggt | 23 | 59.99 | 47.83 | |
| Calpain 2 forward | ttttgtgcggtgtttggtcc | 20 | 59.83 | 50.0 | 107 |
| Calpain 2 reverse | aactcagccacgaagcaagg | 20 | 60.89 | 55.0 | |
| Calpain 3 forward | ttcacaggaggggtgacaga | 20 | 60.11 | 55.0 | 122 |
| Calpain 3 reverse | ttcgtgccatcgtcaatggag | 21 | 61.01 | 52.38 | |
| Calpastatin forward | gccttggatgacctgataga | 20 | 53.8 | 50.0 | 115 |
| Calpastatin reverse | gtgcctcaaggtaggtagaa | 20 | 53.7 | 50.0 |
abp base pair