Fig. 3.

Cyp1b1 gene disruption reduced vascular hypertrophy associated with angiotensin (Ang) II-induced hypertension, which was restored by 6β-hydroxytestosterone (6β-OHT). Mice were infused with vehicle or Ang II (700 ng/kg/day) for 14 days and injected with 6β-OHT (15 μg/g b.w every third day) as described in the “Materials and methods” section. After Ang II infusion, the aorta was removed and processed, H&E staining was performed, and the media/lumen ratio was calculated (a, b). *P < 0.05 vehicle, 6β-OHT vs. corresponding values from Ang II-treated animals; ^†P < 0.05, Cyp1b1^+/+ Ang II vs. Cyp1b1^−/− Ang II (n = 4–5 for all experiments; two-way ANOVA; data are expressed as mean ± SEM)