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. 2020 Jan 16;19:28. doi: 10.1186/s12936-020-3110-6

Table 1.

Working concentrations and sources are provided for the qPCR primers and probes.

Table adapted from Ahmed et al. [17]

Sequence 5′–3′ Concentration (nM) Source
Pan reverse [25] AACCCAAAGACTTTGATTTCTCATAA 200 Eurofins
MAL FP [25] CCGACTAGGTGTTGGATGATAGAGTAAA 50 Eurofins
MAL probe [25] ATTO700-CTATCTAAAAGAAACACTCAT-MGBEDQ 80 Eurogentec
OVA FP [25] CCGACTAGGTTTTGGATGAAAGATTTTT 50 Eurofins
OVA Probe [25] Cy5-CGAAAGGAATTTTCTTATT-MGBEDQ 80 Eurogentec
FAL FP [24] ATTGCTTTTGAGAGGTTTTGTTACTTT 400 Eurofins
FAL RP [24] GCTGTAGTATTCAAACACAATGAACTCAA 400 Eurofins
FAL probe [24] FAM-CATAACAGACGGGTAGTCAT-MGBQ 200 Thermo
VIV FP [24] GCAACGCTTCTAGCTTAATCCAC 400 Eurofins
VIV RP [24] CAAGCCGAAGCAAAGAAAGTCC 400 Eurofins
VIV probe [24] VIC-ACTTTGTGCGCATTTTGCTA-MGBQ 200 Thermo