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Western blotting of constructs for HLA-C*03 and HLA-C*04 indicate variation in protein expression due to alternative splicing. HLA-C allele is indicated at left. The exons present in each splice variant are indicated as per the format a/b/c/1-. HLA-C band intensity was quantified and normalized against actin intensity utilizing ImageJ, shown at the right. The results shown are representative of three independent experiments performed.
