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. 2020 Jan 10;10:3034. doi: 10.3389/fimmu.2019.03034

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Copyright © 2020 Goodson-Gregg, Rothbard, Zhang, Wright, Li, Walker-Sperling, Carrington and Anderson.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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HLA-C*03 and -C*06 expression is similar at low temperature. Growth temperature of JAR cells transfected with HLA-C expression constructs is indicated on the left. The transfected HLA-C*03 a₁/b₁/c₂/1-3 cDNA is indicated as (C*03) and the a₁/b₂/c₂/1-3 HLA-C*06 cDNA is indicated as (C*06), (–) represents cells transfected with a negative control vector. ImageJ actin-normalized HLA-C intensities are quantified and shown at right. Results are reflective of three independent experiments.