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. 2020 Jan 10;10:3034. doi: 10.3389/fimmu.2019.03034

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Copyright © 2020 Goodson-Gregg, Rothbard, Zhang, Wright, Li, Walker-Sperling, Carrington and Anderson.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression levels of HLA-C alleles on peripheral blood NK cells are consistent with the expression level observed by Western blot analysis. The mean fluorescence intensity of HLA-C expression on peripheral blood NK cells is shown for donors that are homozygous for HLA-C*03 (*03/*03) or HLA-C*06 (*06/*06), and heterozygous individuals possessing HLA-C*03 and HLA-C*04 (*03/*04). Statistical significance (p-value < 0.05) is indicated by the asterisks (unpaired t-test). (A) HLA-C intensity for CD56^bright NK cells. (B) HLA-C intensity for CD56^dim NK cells. The *06/*06 individuals have a significantly higher level of HLA-C expression than either the *03/*03 or *03/*04 individuals (*03/*03 vs. *06/*06 p = 0.016, *03/*04 vs. *06/*06 p = 0.0146, unpaired t-test).