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. 2020 Jan 10;7:448. doi: 10.3389/fbioe.2019.00448

Figure 8.

Figure 8

Overview of the 3 cm tibial corticoperiosteal flap results of a pilot study sheep. (A) X- ray image at 12 months' time point. (B) Sagittal plane of the μCT 3D reconstruction. (C) Undecalcified resin section stained with Goldner's trichrome. (D–F) Scanning electron microscopic (SEM) images of newly formed bone and interface with host bone. (D) Interface of the host bone (HB) with the new formed bone (NB) indicating excellent osteointegration of host bone, scaffold (SC) and the corticoperiosteal flap. (E) Higher magnification image showing integration of the corticoperiosteal within the newly formed bone and (F) higher magnification of the white square box in image (E) showing two osteocytes embedded in the new formed bone and directly attached to the corticoperiosteal flap, indicating direct interaction of corticoperiosteal with osteocytes. Fissures in images (D,E) are artifacts resulting from sample preparation. - - - Defect site; *Mechanical testing artifact; Scale bar: (B) 5 mm; (C) 5 mm; (D) 200 μm; (E) 100 μm and (F) 10 μm.