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. 2019 Dec 16;43(2):525–535. doi: 10.3892/or.2019.7438

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Copyright: © Cui et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — miR-144 is a target of XIST in LSCC cells. (A) The predicted binding and mutant sites between miR-144 and XIST are presented. (B) Luciferase activity was detected in TU212 cells following co-transfection with miR-144/miR-NC mimic and Wt-XIST or Mut-XIST reported plasmid. (C) miR-144 expression was assessed in TU212 cells transfected with sh-XIST or sh-NC by RT-qPCR. (D) XIST expression was assessed in TU212 cells transfected with miR-144/miR-NC or miR-144 inhibitor/anti-miR-NC by RT-qPCR. (E) miR-144 expression was examined in TU212 cells transfected with miR-144/miR-NC or miR-144 inhibitor/anti-miR-NC by RT. (F) miR-144 expression was examined in LSCC tissues and adjacent normal tissues (n=48) by RT-qPCR. (G) Correlation between XIST expression and miR-144 expression in LSCC tissues was analyzed by Pearson's correlation analysis. **P<0.01. Wt, wild-type; Mut, mutant-type; RT-qPCR, reverse transcription-quantitative PCR; LSCC, laryngeal squamous cell carcinoma; XIST, X inactive-specific transcript; NC, negative control.

Figure 4. — miR-144 is a target of XIST in LSCC cells. (A) The predicted binding and mutant sites between miR-144 and XIST are presented. (B) Luciferase activity was detected in TU212 cells following co-transfection with miR-144/miR-NC mimic and Wt-XIST or Mut-XIST reported plasmid. (C) miR-144 expression was assessed in TU212 cells transfected with sh-XIST or sh-NC by RT-qPCR. (D) XIST expression was assessed in TU212 cells transfected with miR-144/miR-NC or miR-144 inhibitor/anti-miR-NC by RT-qPCR. (E) miR-144 expression was examined in TU212 cells transfected with miR-144/miR-NC or miR-144 inhibitor/anti-miR-NC by RT. (F) miR-144 expression was examined in LSCC tissues and adjacent normal tissues (n=48) by RT-qPCR. (G) Correlation between XIST expression and miR-144 expression in LSCC tissues was analyzed by Pearson's correlation analysis. **P<0.01. Wt, wild-type; Mut, mutant-type; RT-qPCR, reverse transcription-quantitative PCR; LSCC, laryngeal squamous cell carcinoma; XIST, X inactive-specific transcript; NC, negative control.