Figure 2.
eIF4E is upregulated, whereas miR-320a and miR-340-5p are downregulated in HEC-1A and RL95-2 cells. (A and B) eIF4E expression levels in HEC-1A, Ishikawa, and RL95-2 cells were determined by western blotting. (C) miR-320a expression levels in HEC-1A, Ishikawa and RL95-2 cells were determined by RT-qPCR. (D) miR-340-5p expression levels in HEC-1A, Ishikawa and RL95-2 cells were determined by RT-qPCR. *P<0.05 and **P<0.01. eIF4E, eukaryotic translation initiation factor 4E; miR, microRNA; RT-qPCR, reverse transcription-quantitative PCR.