Figure 6.
miR-320a and miR-340-5p mimic treatment suppresses the expression of MMP-3 and MMP-9 in HEC-1A cells. (A) Western blotting analysis indicated that the expression levels of MMP-3 and MMP-9 were downregulated following miR-320a or miR-340-5p mimic treatment. (B-E) Quantification of (B) MMP-3, (C) MMP-9, (D) p-eIF4E and (E) eIF4E relative expression levels (n=3). *P<0.05, **P<0.01, ***P<0.001 and ****P<0.0001. eIF4E, eukaryotic translation initiation factor 4E; p, phosphorylated; miR, microRNA; MMP, matrix metallopeptidase; negative, mock transfections; scrambled, cells treated with scrambled-oligomer control RNA; miR-320a or miR-340-5p, cells treated with miR-320a or miR-340-5p mimics.