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. 2019 Dec 13;43(2):427–436. doi: 10.3892/or.2019.7434

Figure 1.

Figure 1.

Expression of KLF9 is increased by Dex in RAW264.7 cells. (A) Real-time quantitative PCR analysis of the mRNA level of KLF9 in RAW264.7 cells 16 h after treatment with saline or Dex (100 nM) (n=5). (B) Western blot analysis of KLF9 in RAW264.7 cells as described in A; quantitative data are on the right. (C) A series of promoter activity of KLF9 was detected in RAW264.7 cells with saline or Dex (100 nM) treatment. (D) A ChIP assay was performed using anti-GR antibody in RAW264.7 cells. (E) Real-time quantitative PCR analysis of KLF9 in RAW264.7 cells treated with 100 nM Dex or with 10 µM of the GR antagonist RU486 for 16 h. (F) Western blot analysis of the protein level of KLF9 described in E; quantitative data are on the right. Experiments were performed in triplicate. Error bars represent the standard deviation *P<0.05; **P<0.01; ***P<0.005; ****P<0.001. KLF9, Krüppel-like factor 9; Dex, dexamethasone; ChIP, chromatin immunoprecipitation; GR, glucocorticoid receptor.