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. 2019 Dec 27;43(2):549–561. doi: 10.3892/or.2019.7449

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Copyright: © Lee et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Niclosamide treatment induces apoptosis in esophageal cancer cell lines. (A) BE3 cells were treated with 10 µM niclosamide, then harvested at different time-points after treatment, or (B) treated with various doses of niclosamide for 48 h. Treated cells were lysed for western blot analysis of PARP. The position of the native PARP (f-PARP) and the cleaved fragment (c-PARP) is indicated. (C) CE48T, (D) CE81T and (E) BE3 cells were treated with 10 µM niclosamide for 48 h and the apoptosis levels were detected by Annexin V/PI staining. DMSO treatment was used as the control. The proportion of Annexin V⁺/PI⁻ and Annexin V⁺/PI⁺ cells indicated the early apoptotic and dead cells, respectively. Results were expressed as the means ± SD of three independent experiments. *P<0.05, **P<0.005, and ***P<0.0005. PARP, poly(ADP-ribose) polymerase.