Figure 4.

MTS assays of esophageal cancer cells treated with a chemotherapeutic drug alone or in combination with niclosamide. Esophageal cancer cells (A) CE48T, (B) CE81T and (C) BE3 cells were seeded in 96-well plates and treated for 72 h with serial doses of 5-FU, cisplatin, paclitaxel, or in combination with 2.5 µM of niclosamide. Cell viability was then analyzed by MTS assay. F, C, and P indicate the treatment of 5-FU, cisplatin, or paclitaxel, respectively. F+N, C+N, and P+N indicate the combination treatment of niclosamide with 5-FU, cisplatin, or paclitaxel, respectively. 5-FU, 5-fluorouracil.