Figure 5.

Cell apoptosis assay of CE48T cells using Annexin V/PI double-staining method. (A) CE48T cells were treated with niclosamide (2.5 µM), 5-FU (50 µM), cisplatin (10 µM), paclitaxel (10 nM) alone, or in combination with niclosamide and each of these chemotherapeutic drugs for 48 h. The apoptotic levels were detected by Annexin V/PI staining and analyzed by flow cytometric assay. DMSO treatment was used as the control. The proportion of Annexin V (+) cells indicate the stage of apoptosis. (B) The percentage of Annexin V (+) cells were expressed as the means ± SD of three independent experiments. *P<0.05 and **P<0.005. 5-FU, 5-fluorouracil.