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. 2019 Dec 11;43(2):415–426. doi: 10.3892/or.2019.7427

Figure 1.

Figure 1.

Effects of Evo on HCT116 cell proliferation. (A) CCK-8 assay results revealed the anti-proliferative effect of Evo in HCT116 cells (*P<0.05 and **P<0.01 vs. the control group). (B) Colony formation results revealed the anti-proliferative effect of Evo in HCT116 cells. (C) Western blot analysis results revealed the effect of Evo on the protein expression of PCNA in HCT116 cells; GAPDH was used as a loading control. (D) Quantification of the western blot analysis results revealed the effect of Evo on the protein expression of PCNA in HCT116 cells (**P<0.01 vs. the control group). (E) Flow cytometric results revealing the effect of Evo on cell cycle arrest in HCT116 cells. Evo, evodiamine.