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. 2019 Dec 11;43(2):415–426. doi: 10.3892/or.2019.7427

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 6. — Effects of Evo, BMP9 and/or HIF-1α on the activity of p53 in HCT116 cells. (A) Western blot analysis results revealed the effect of Evo on p53 and p-p53 in HCT116 cells; GAPDH was used as the loading control. (B) Quantification of the western blot analysis results revealed the effect of Evo on p53 and p-p53 in HCT116 cells (*P<0.05 and **P<0.01 vs. the control group). (C) Western blot analysis results revealed the effect of Evo and/or BMP9 on p53 and p-p53 in HCT116 cells; GAPDH was used as the loading control. (D) Quantification of the western blot analysis results revealed the effect of Evo and/or BMP9 on the protein expression of p53 and p-p53 in HCT116 cells (**P<0.01 vs. the control group; ^##P<0.01 vs. the Evo treated group). (E) Western blot analysis results revealed the effect of Evo and/or BMP9 silencing on p53 and p-p53 in HCT116 cells; GAPDH was used as the loading control. (F) Quantification of the western blot analysis results revealed the effect of Evo and/or BMP9 silencing on the protein expression of p53 and p-p53 in HCT116 cells (**P<0.01 vs. the control group; ^##P<0.01 vs. the Evo treated group). (G) Western blot analysis results revealed the effect of Evo and/or HIF-1α on p53 and p-p53 in HCT116 cells; GAPDH was used as the loading control. (H) Quantification of the western blot analysis results revealed the effect of Evo and/or HIF-1α on the protein expression of p53 and p-p53 in HCT116 cells (*P<0.05 and **P<0.01 vs. the control group; ^##P<0.01 vs. the Evo treated group). (I) Western blot analysis results revealed the effect of Evo and/or HIF-1α silencing on p53 and p-p53 in HCT116 cells; GAPDH was used as the loading control. (J) Quantification of the western blot analysis results revealed the effect of Evo and/or HIF-1α silencing on the protein expression of p53 and p-p53 in HCT116 cells (*P<0.05 and **P<0.01 vs. the control group; ^##P<0.01 vs. the Evo treated group). Evo, evodiamine; BMP9, bone morphogenetic protein 9.

Figure 6. — Effects of Evo, BMP9 and/or HIF-1α on the activity of p53 in HCT116 cells. (A) Western blot analysis results revealed the effect of Evo on p53 and p-p53 in HCT116 cells; GAPDH was used as the loading control. (B) Quantification of the western blot analysis results revealed the effect of Evo on p53 and p-p53 in HCT116 cells (*P<0.05 and **P<0.01 vs. the control group). (C) Western blot analysis results revealed the effect of Evo and/or BMP9 on p53 and p-p53 in HCT116 cells; GAPDH was used as the loading control. (D) Quantification of the western blot analysis results revealed the effect of Evo and/or BMP9 on the protein expression of p53 and p-p53 in HCT116 cells (**P<0.01 vs. the control group; ^##P<0.01 vs. the Evo treated group). (E) Western blot analysis results revealed the effect of Evo and/or BMP9 silencing on p53 and p-p53 in HCT116 cells; GAPDH was used as the loading control. (F) Quantification of the western blot analysis results revealed the effect of Evo and/or BMP9 silencing on the protein expression of p53 and p-p53 in HCT116 cells (**P<0.01 vs. the control group; ^##P<0.01 vs. the Evo treated group). (G) Western blot analysis results revealed the effect of Evo and/or HIF-1α on p53 and p-p53 in HCT116 cells; GAPDH was used as the loading control. (H) Quantification of the western blot analysis results revealed the effect of Evo and/or HIF-1α on the protein expression of p53 and p-p53 in HCT116 cells (*P<0.05 and **P<0.01 vs. the control group; ^##P<0.01 vs. the Evo treated group). (I) Western blot analysis results revealed the effect of Evo and/or HIF-1α silencing on p53 and p-p53 in HCT116 cells; GAPDH was used as the loading control. (J) Quantification of the western blot analysis results revealed the effect of Evo and/or HIF-1α silencing on the protein expression of p53 and p-p53 in HCT116 cells (*P<0.05 and **P<0.01 vs. the control group; ^##P<0.01 vs. the Evo treated group). Evo, evodiamine; BMP9, bone morphogenetic protein 9.