Figure 3.

Stage-specificity and transmission blocking potential of amino-artemisinins against stage IV/V and stage V gametocytes. (A) Population compositions at time of treatment (0 h) for both stage III/IV/V (10% stage III, 50% stage IV, 40% stage V) or mature stage V (>95% stage V) gametocytes were determined microscopically using Giemsa stained smears (n ≥ 1,000 infected erythrocytes were counted). (B) Box-plot of mean IC₅₀ values of amino-artemisinins after 72 h and 72 + 24 h treatment for both stage III/IV/V and V gametocytes. Dose-responses of DHA 2 and amino-artemisinins against a (C) stage III/IV/V and (D) stage V population using the luciferase reporter line exposed to 10-fold serial dilutions for 72 h at 37°C under 90% N₂, 5% CO₂, and 5% O₂ atmospheric conditions. Data are averages of for a single independent biological experiment, performed in technical triplicates, error bars indicate ± SD.