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. 2020 Jan 10;10:1650. doi: 10.3389/fpls.2019.01650

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Copyright © 2020 Wei, Mao, Lu, Wei, Han, Guan, Yang, Zha, Xu and Luo

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The AchnMYC2, AchnMYB41, and AchnMYB107 individually activate the AchnCYP86A1 promoter. (A) Yeast one-hybrid assay of AchnMYC2, AchnMYB41 and AchnMYB107 to the AchnCYP86A1 promoter. AD-Rec-p53 with p53-AbAi was used as a positive control, while AD-empty with AchnCYP86A1-AbAi was used as a negative control. (B) AchnMYC2, AchnMYB41 and AchnMYB107 activate the AchnCYP86A1 promoter in dual-luciferase assays. The ratio of LUC/REN of the empty vector (SK) plus promoter was used as calibrator (set as 1). Pro, promoter; Ept, empty. Error bar represents standard deviation of three independent experiments. Asterisk indicates significant difference (t test, *p value < 0.05).