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. 2020 Jan 10;10:3057. doi: 10.3389/fimmu.2019.03057

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Copyright © 2020 Laparidou, Schlickenrieder, Thoma, Lengyel and Schusser.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Gene editing of CXCR4 with the CRISPR/Cas9 system in chicken DT40 cells. (A) CXCR4 gene structure with guide RNA (gRNA) recognition site and protospacer-adjacent motif (PAM) sequence. (B) Sequence analysis of CXCR4^neg and wt DT40 cells with amino acid sequence. CXCR4^neg cell sequence analysis revealed a T insertion causing a frameshift and therefore generation of a premature stop codon. (C) Flow cytometry analysis of CXCR4^neg and wt cells with staining for CXCR4. Gene editing successfully knocked out the CXCR4 chemokine receptor.