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. 2020 Jan 10;10:3057. doi: 10.3389/fimmu.2019.03057

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Copyright © 2020 Laparidou, Schlickenrieder, Thoma, Lengyel and Schusser.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CXCR4/CXCL12 dependent migration of DT40 cells and primary bursal cells. The DT40 cell migration seen in chemotaxis assays was inhibited by (A) knocking out the CXCR4 receptor in the DT40 cell line (A), Treating the cells with the CXCR4 antagonist, AMD3100 also led to cell migration inhibition (B). Finally, DT40 cells lacking the B cell receptor migrated in much lower levels toward the chemokine (C) CXCL12 in all cases n ≥ 3, data normally distributed per Kolmogorov-Smirnov and Shapiro-Wilk tests, independent t-test analysis, *p < 0.05 in case of (A,B) and p > 0.05 in case of (C). In addition chemotaxis assays using primary bursal B cells were performed as described before. The migration of the BCR^neg cells toward CXCL12 was compromised as seen in (D). While 81% of the wt cells migrated toward CXCL12 only 8% of the BCR^neg cells migrated toward CXCL12. One representative FACS analysis is shown.