Figure 3. Endpoint analysis of combination treatments synergistically induced anti-proliferation in 3 sarcoma cell lines.

A representative MTT endpoint graph is displayed for HOS (A) after 48 hours as well as HT1080 (B) and SW982 (C) after 72 hours of contact with PENAO, gefitinib and fixed ratios of combination treatment. (D) Combination index (CI) and dose reduction index (DRI) values were determined for 3 sarcoma cell lines using CalcuSyn software. Effective dose (ED_X) is the amount of drug required to produce an effect on X% of the cell population. Dose reduction index (DRI) is the measure of fold each treatment in a synergistic combination can be reduced. Results are presented as a mean ± standard deviation. Graphs displayed are representative of at least 3 experiments performed in triplicate samples for each treatment.