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(A) Scheme for the sandwich ELISA (sELISA) with soluble HER3-GFP proteins for the selection of anti-HER3 mAbs. (B) An example of first screening of anti-HER3 mAbs using sELISA. (C) Second and third FCM screenings of anti-HER3 mAbs using HEK293 transfectants expressing GFP-fused HER family proteins. (D) Representative FCM histograms showing the reactivity of anti-HER3 mAb (Ab4) with human cancer cell lines.
