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. 2020 Jan 7;11(1):31–45. doi: 10.18632/oncotarget.27414

Figure 5. Internalization activity and inhibition of NRG1 binding to HER3 by anti-HER3 mAbs.

Figure 5

(A) Scheme for the internalization of HER3 by mAbs. Human cancer cells were treated with anti-HER3 mAbs at 37° C or 4° C for 1~1.5 h, and cell-surface HER3 proteins were analyzed by FCM. (B) Internalization of cell-surface HER3 proteins of LS-174T and T47D by anti-HER3 mAbs was shown as the internalization (%). (C) Scheme for the binding inhibition of mAbs to HEK293 cells expressing HER3-GFP in the presence of NRG1 (upper). Representative binding of anti-HER3 mAb in the absence (-) or presence (+) of NRG1(lower). (D) Histogram (left) for the binding of mAb to HEK293 cells expressing HER3-GFP in NRG1(+) or NRG1(-). Bar graph (right) shows binding inhibition of anti-HER3 mAbs with HEK293 cells expressing HER3-GFP in NRG (+).