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. 2020 Jan 10;12:326. doi: 10.3389/fnmol.2019.00326

Table 3.

Sequences of primers used for qRT-PCR assay.

Gene name Primer sequence Ta Opt (°C) Product size(bp)
U6 F:5′ GCTTCGGCAGCACATATACTAAAAT 3′ R:5′ CGCTTCACGAATTTGCGTGTCAT 3′ 60 89
β-actin F:5′ CGAGTACAACCTTCTTGCAGC 3′ R :5′ ACCCATACCCACCATCACAC 3′ 60 202
tiRNA-Gly-GCC-001 F:5′ GCATGGGTGGTTCAGTGGTAG 3′ R:5′ ACGTGTGCTCTTCCGATCTCA 3′ 60 52
tRF-Gly-GCC-012 F:5′ GCATGGGTGGTTCAGTGGTAG 3′ R:5′ CGTGTGCTCTTCCGATCTGA 3′ 60 46
tRF-Gly-GCC-013 F:5′ GCATGGGTGGTTCAGTGGTAG 3′ R:5′ CGTGTGCTCTTCCGATCTCG 3′ 60 47
tRF-Gly-GCC-016 F:5′ GCATGGGTGGTTCAGTGGTAG 3′ R:5′ ACGTGTGCTCTTCCGATCTAGG 3′ 60 51
BDNF F:5′ GCGTGTGTGACAGTATTAGCGAG 3′ R:5′ GGCATTGCGAGTTCCAGTG 3′ 60 210