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MV uptake in isolated perfused lungs (IPL). Lungs from normal or low-dose LPS-treated (20 ng i.v., 2 h) mice were perfused and mechanically ventilated using the IPL system. After a brief, slow flow-rate flush (5 min) to remove non-marginated cells, DiD-labelled MVs (240,000 FU) were infused into the perfusion circuit and recirculated for 1 h followed by determination of cell-associated DiD levels (MFI). Data are displayed as mean ± SD and analysed by t-tests. n = 3, *p < 0.05.
