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. 2020 Jan 5;9(1):1706708. doi: 10.1080/20013078.2019.1706708

Figure 7.

Figure 7.

Receptor-dependent mechanisms of MV uptake by lung-marginated Ly6Chigh monocytes. (a) & (b): In vivo expression of PS and scavenger receptors on monocyte subsets were determined in lung single cell suspensions from normal and LPS-treated mice (20 ng, i.v. 2 h) by flow cytometry. (c): MV uptake by lung Ly6Chigh monocytes isolated by perfusion of individual LPS-treated mice was assessed in the presence of PS liposomes (PS-lipo) or RGDS peptide, in comparison to the respective controls, PC liposomes (PC-lipo) and RGES peptide. (d): MV uptake by lung Ly6Chigh monocytes from individual LPS-treated mice was similarly assessed in the presence of scavenger receptor A blockers: polyinosinic acid (Poly-I) and dextran sulphate (D-S), in comparison to the respective controls, polycytidylic acid (Poly-C) and chondroitin sulphate (C-S). Data are displayed as mean ± SD and analysed by multiple t-tests (a,b), one-way ANOVA with Bonferroni correction tests ((c), peptide blocking), or as median ± IQR and analysed by Friedman with Dunn’s correction tests ((c), liposome blocking; (d)). n = 3 for receptor expression and n = 3–5 for blocking experiments.