Figure 8.

Uptake of circulating PS liposomes during low-grade systemic inflammation. DiD-labelled liposomes were injected i.v. into untreated or low-dose LPS-treated (20 ng, i.v. 2 h) mice. At 1 h post-MV injection, lungs (a), liver (b) and spleen (c) were harvested for preparation of fixed single-cell suspensions and analysis by flow cytometry. Cell-associated DiD fluorescence is indicated (MFI) as a measure of liposome uptake per cell in each of the cell populations. Total uptake of PS-liposomes and per each organ type (d–f) was estimated (see Methods) by calculating the individual mouse cell-associated DiD MFI values (a–c) × group mean cell counts/organ (data in Figure 3). Data are displayed as mean ± SD and analysed by two-way ANOVA with Bonferroni correction tests. n = 5, **p < 0.01, ***p < 0.001.