Figure 2.

Isolation and characterization of extracellular vesicles from control astrocytes and IL-1β-treated astrocytes. (a) The yield of IL-1β-ADEVs, expressed as the number of isolated particles per mL media, was significantly higher than that of CTL-ADEVs (n = 5). (b) The nanogram protein of EVs per mL media (c) and the amount of protein per particle was not significantly different between the two groups (n = 5). (d) Representation of size distribution of CTL- and IL-1β-ADEVs by nanoparticle tracking analysis (n = 5). Data are expressed as mean ± SEM. n.s., no significance; *p < 0.05 compared to control group as determined by student’s t-test using Graphpad prism 6.