Table 2. Epitope mapping of N9 mAbs site-directed mutant NAs from H7N9 SH/2/13 by IFAs.
MAbs | Mutant sitea | IFAb |
---|---|---|
5F2 | A370T | − |
S371L | − | |
WT | + | |
2H10 | D436E | − |
K437R | − | |
WT | + | |
7D8 | P368S | − |
T369L | − | |
WT | + | |
7H2 | H151P | − |
N200S | − | |
WT | + | |
D3 | A248G | − |
T249N | − | |
WT | + | |
F6 | N346S | − |
N348S | − | |
WT | + | |
B7C2 | A401G | − |
W403K | − | |
WT | + |
aThe amino acid mutant positions were in N9 numbering.
bIFAs were performed on 293 T cells transfected with mutant NA constructions. “+” or “−” indicated the presence or absence of fluorescence.