TABLE 3.
Strain | MBC (μg/ml)a
|
|||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Chlorhexidine |
Chlorhexidine digluconate |
Benzalkonium chloride |
Cetrimide |
Pentamidine isethionate |
Ethidium bromide |
|||||||
Median | Max | Median | Max | Median | Max | Median | Max | Median | Max | Median | Max | |
RN4220 | 3.2 | 3.2 | 2 | 8 | 8 | 8 | 4 | 4 | 25 | 50 | 16 | 32 |
RN4220 ΔnorA | 3.2 | 3.2 | 4 | 4 | 8 | 8 | 2 | 2 | 3.1 | 3.1 | 2 | 8 |
RN4220 ΔnorA (pCN51-norA) | 3.2 | 3.2 | 4 | 4 | 8 | 8 | 8 | 8 | 50 | 50 | 16 | 16 |
RN4220 N1 | 3.2 | 3.2 | 4 | 4 | 8 | 8 | 4 | 8 | 50 | 50 | 32 | 32 |
RN4220 N2 | 3.2 | 3.2 | 8 | 16 | 16 | 16 | 8 | 16 | 100 | 100 | 32 | 64 |
RN4220 (pCN38) | 3.2 | 3.2 | 4 | 4 | 4 | 8 | 2 | 4 | 25 | 25 | 16 | 16 |
RN4220 (pCN38-qacC [pSK89]) | 3.2 | 3.2 | 2 | 4 | 8 | 8 | 4 | 4 | 25 | 25 | 32 | 32 |
RN4220 (pCN38-qacC [pSK41]) | 3.2 | 3.2 | 4 | 8 | 32 | 32 | 8 | 16 | 25 | 25 | 256 | 256 |
RN4220 (pCN38-qacA qacR) | 3.2 | 3.2 | 4 | 8 | 16 | 16 | 8 | 16 | 400 | 800 | 256 | 256 |
RN4220 ΔnorA (pCN38-qacA qacR) | 3.2 | 3.2 | 8 | 16 | 16 | 32 | 4 | 8 | 400 | 400 | 128 | 128 |
RN4220 (pCN38-qacA qacR qacC [pSK41]) | 3.2 | 6.4 | 8 | 8 | 16 | 16 | 16 | 16 | 200 | 200 | 256 | 256 |
RN4220 (pCN38-qacA qacR qacC [pSK89]) | 3.2 | 3.2 | 8 | 8 | 32 | 64 | 16 | 16 | 200 | 200 | 128 | 128 |
RN4220 N1 (pCN38-qacA qacR) | 6.4 | 6.4 | 4 | 8 | 16 | 16 | 16 | 16 | 400 | 400 | 256 | 256 |
S. aureus 2014.C02 | 3.2 | 3.2 | 8 | 8 | 16 | 16 | 8 | 16 | 400 | 800 | 256 | 512 |
Values are from 3 independent replicates. Values in italics indicate an MBC less than that of the wild type. Bold values indicate an MBC greater than that of the wild type.