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. 2020 Jan 10;10:3010. doi: 10.3389/fmicb.2019.03010

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Copyright © 2020 Kang, Jacquiod, Herschend, Wei, Nesme and Sørensen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Integrated comparison of selected SMC. (A) 21 SMC divided into seven groups based on their community similarities from 16S rRNA gene analysis (weighted UniFrac distance). Group 1 (Gr1) includes three simplified microbial consortia SMC1, SMC2, and SMC3 from dilution 10^–8. A total of 18 SMC (SMC4 – SMC21) were clustered into six groups (Gr2 – Gr7) from dilution 10^–9. (B) Cell density (OD_600nm) comparison of group 1–7, KMCG6 and the most abundant species in the initial KMCG6, Chryseobacterium sp. KMC2 (CKMC2). (C) Comparison of enzymes (protease and keratinase) activity. (D) Comparison of the residual substrate ratio. Lowercase letters (e.g., a, b, and c) in (B–D) refer to significant differences between groups with one-way ANOVA followed by post hoc Tukey’s HSD test (p < 0.05).