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Gene deletion construct and identification of deletion mutants. (A) MoPAS1 was deleted through the targeted gene replacement method; (B) Confirmation of MoPAS1 deletion using Southern blot analysis. DNA samples were digested with SacI. A 1.9-kb band from wild-type KJ201 and a 3.4-kb band from knock-out mutants were produced. Lane 1, wild-type; lane 2–3, knock-out mutants; (C) Confirmation of MoPAS1 deletion and complemented transformant Mopas1c using RT-PCR.
