Fig. 1.

High-throughput microscope with temporally-coded illumination. A) Our system consists of an inverted wide-field fluorescence microscope with a 2-axis motion stage and a programmable LED illumination source. The sample is scanned at a constant speed while being illuminated with temporally coded illumination pulses during each exposure. The excitation wavelength filter may be removed for conventional brightfield imaging. B) Illustration of the pipeline from sample and acquired images to multi-frame reconstruction. C) Image of our system - a Nikon TE300 microscope configured with a Prior motion stage and LED illuminator [27].