Figure 5.

Inhibition of TRIM32 induced by miR-519d suppresses Bcl-2 expression and promotes the cisplatin-induced generation of ROS. (A) Effect of miR-519d and TRIM32 plasmid on changing the Bcl-2 expression was evaluated by Western blot assay. (B) SW480 and HT29 cells were transfected with miR-519d and TRIM32 plasmid. 24 hrs later, these cells were treated with cisplatin (1 μM) for another 48 hrs. Flow cytometry analysis was performed to detect the generation of ROS.

Notes: Data was expressed as mean±SD. *P<0.05 vs NCO group, ^#P<0.05 vs cisplatin + NCO group, ^&P<0.05 vs cisplatin + miR-519d group.

Abbreviations: TRIM32, tripartite motif 32; ROS, reactive oxygen species.