Figure 2.

CA6 induces ROS-dependent G2/M cell cycle arrest. (A) BGC-823 (first row) and SGC-7901 (second row) were challenged with CA6 for 16 h, with or without pretreatment with NAC (5 mM) for 2 h. Cell cycle distribution was analyzed by PI staining. Representative histograms are shown [n = 3]. (B and C) Quantification of cells in the G2/M phase cells following exposure to CA6. Cells were treated as indicated in panel A [Mean ± SEM; n = 3; *P<0.05, **P<0.01, ***P < 0.001]. (D) BGC-823 and SGC-7901 cells were exposed to 15 µM CA6 for indicated time points. Levels of cell cycle regulating proteins Cyclin B1, MDM-2 and CDC-2 were determined by Western blot. Curcumin (20 µM) was used as the positive control and GAPDH as the loading control. Representative blots shown [n = 3]. (E) BGC-823 and SGC-7901 cells were exposed to CA6, with or without NAC pretreatment (5 mM). Levels of Cyclin B1, MDM-2 and CDC-2 were determined. GAPDH was used as loading control. Representative blots shown [n = 3].