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. 2019 Dec 4;15(12):e1008152. doi: 10.1371/journal.ppat.1008152

Fig 1. Determination of ROD21 excision from S. Enteritidis chromosome during the infective cycle.

Fig 1

After intragastric (i.g.) infection of C57BL/6 mice, quantification of total Salmonella gDets and absolute ROD21 excision in different portions of the gastrointestinal tract and deep organs was performed by the quantification of the invA (total bacteria, empty bars) or attB (excised bacteria, black bars) sequences, by qPCR. gDNA from small intestine (A), caecum (B), colon (C), mLN (D), spleen (E) and liver (F) were purified and the presence of S. Enteritidis-WT chromosomes was identified, number of copies for each sequence were normalized per μg of gDNA. Empty circles and dotted line over each time post-infection are the relative ROD21 excision value (REROD21 = attB gDets/ invA gDets). The assay included 4 mice for each time. 2-way ANOVA with Tukey´s post-test α = 0.05; *p<0.05, **p<0.005, ***p<0.0005, ****p<0.0001.