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. 2019 Dec 4;15(12):e1008152. doi: 10.1371/journal.ppat.1008152

Fig 4. ROD21 excision is required for the intracellular colonization of S. Enteritidis in the intestinal epithelium during the infective cycle.

Fig 4

C57BL/6 mice were i.g. infected with 1x106 CFU of S. Enteritidis-WT (black circles), S. Enteritidis-ΔROD21 (grey circles), S. Enteritidis-ΔROD21::ROD21 (black diamonds), S. Enteritidis-ΔDRSΔint (grey diamonds) or S. Enteritidis-ΔintΔrdf (empty triangles). At 48 and 144 hpi a gentamicin protection assay was performed and intracellular loads were evaluated on small intestine (A), colon (B), mLN (C), blood (D), spleen (E) and liver (F). The assay included 6 mice at all times post infection. Comparisons of bacterial loads between different times post-infection were analyzed by 2-way ANOVA with Tukey´s post-test α = 0.05. *p<0.05, **p<0.005, ***p<0.0005, ****p<0.0001.