Primers and probes to test ROD21 genes |
Gene |
Primer exp Fw (Sequence 5´ to 3´) |
Primer exp Rv (Sequence 5´ to 3´) |
Probe |
Hypothetical role |
SEN1970 |
GCC ATG CGG ATT AAG CAA TAC |
TTT TGC TGG ACG GCA TGA C |
TCA CCG CGA TCC TA30
|
Integrase. P4-like integrase. |
SEN1975 |
GAA TTA AAT GGT TTG ACT GCT AGA GAG A |
TCG TGC CAG ATA GGC AGT ATT ACT |
AGC GGT GAA AAC C30
|
TlpA. Cytoplasmic protein with TIR domain (Salmonella sp.) Identities: 100%. |
SEN1976 |
GCC GAT CCC GGA CAA GA |
AGT ATC GGG CTA TAA GTG TGT AAT ACC A |
CAT CCC CTT GTA TCG CT30
|
Pseudogene (putative type IV prepilin protein, S. Gallinarum. Identities: 99%). |
SEN1979 |
GGC TAC TTC TGC CCC AAG AA |
GTC AAT GGA GAA ATC AGA ACA AAA GA |
ATC TTA AGC CTT GTT TCA AC30
|
Conserved hypothetical protein (S. Enteritidis and S. Dublin). Identities: 100%. (Conjugal transfer protein TraD domain). |
SEN1980 |
TGG TCA TAT TAT ATT GGC ACC TGT TT |
CAC AAT GCT GAG GAG CTT TCA A |
CGC ACC TGC TCA TAT30
|
MobA/MobL family protein (S. Dublin) and possible conjugal transfer protein (S. Enteritidis). Identities: 100%. (MobA/MobL family). |
SEN1982 |
CCA TCA GGC CCT GCT CAA |
TGG CAC AGG GAC GAA TTC A |
AAA ACC GTC GAG GAT C30
|
Lipoprotein (S. Enteritidis). Identities: 100%. |
SEN1993 |
GCA GAA CTC CTT GCT GCT GTT |
GAG CGC GGG TCG ATT TG |
CTC ATC CCA ACC TAA AA30
|
DNA-binding protein (histone-like protein hlp-II) (S. Gallinarum and S. Enteritidis). Identities: 100% (Domain: global DNA-binding transcriptional dual regulator H-NS; Provisional). |
SEN1994 |
AGT GCC TTT TGA GCC ATT GAA |
TGC AAT TTA TAT CGG AGC ACC AT |
ATG CCT TGT AAT TCG TG30
|
Membrane protein (S. Gallinarum and S. Enteritidis). Identities: 100%. |
SEN1998 |
GAA AAC GCC CGG CCT TAA |
CGC ACC GGA TTT GGT AAA A |
CAG TTC GTA AAT CCA C30
|
Phage regulatory protein (Salmonella sp.). Identities: 100%. (Domain: Prophage CP4-57 regulatory protein (AlpA)/Predicted transcriptional regulator (Blum et al.)). |