Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Danio rerio) | gdf6a(lf) |
Gosse and Baier, 2009
PMID: 19164594 |
gdf6as327 allele | |
| Strain, strain background (Danio rerio) | gdf6b(lf) | This paper | Generated using Golden Gate TALEN kit – see Materials and methods | |
| Strain, strain background (Danio rerio) | Tg(mitfa:eGFP) |
Curran et al., 2009
PMID: 19527705 |
||
| Strain, strain background (Danio rerio) | Tg(crestin:eGFP) | Kaufman et al., 2016 -- PMID:26823433 | ||
| Strain, strain background (Danio rerio) | Tg(crestin:mCherry) |
Kaufman et al., 2016
PMID:26823433 |
||
| Strain, strain background (Danio rerio) |
Tg(ubi:switch) |
Mosimann et al., 2011
PMID: 21138979 |
||
| Strain, strain background (Danio rerio) | Tg(mitfa:gdf6a) | This paper |
gdf6a expressed under the mitfa promoter, generated using Tol2Mediate transgenesis – see Materials and methods. |
|
| Strain, strain background (Danio rerio) | mitfa(lf) |
Lister et al., 1999
PMID: 10433906 |
nacre mutant | |
| Strain, strain background (Danio rerio) | foxd3(lf) |
Stewart et al., 2006
PMID: 16499899 |
sym1 mutant | |
| Antibody | Phospho-SMAD1/5/9, rabbit monoclonal | CellSignaling | #13820 | 1:200 |
| Antibody | Mitfa, rabbit polyclonal | Venkatesan et al. | N/A | 1:100 |
| Antibody | GFP, mouse monoclonal | Thermo-Fisher | #MA5-15256 | 1:500 |
| Antibody | Goat anti-Mouse IgG-Alexafluor 488 | Thermo-Fisher | #A-11001 | 1:300 |
| Antibody | Goat anti-Rabbit IgG-Alexafluor 555 | Thermo-Fisher | #A-21428 | 1:300 |
| Antibody | Anti-DIG-AP Fab Fragments | Roche | #11093274910 | 1:1000 |
| Antibody | HuC/HuD antibody, mouse monoclonal | Thermo-Fisher | #A-21271 | 1:100 |
| Recombinant DNA reagent | pENTRP4P1r-mitfa | Ceol et al., 2011 -- PMID: 21430779 | ||
| Recombinant DNA reagent | pDONR221-gdf6b | Venkatesan et al., 2018-- PMID: 29202482 | ||
| Recombinant DNA reagent | pDONR221-gdf6a | This paper | Tol2 p221 entry vector containing gdf6a | |
| Recombinant DNA reagent | pDONR221-Cre-ERT2 |
Mosimann et al., 2011
PMID: 21138979 |
||
| Recombinant DNA reagent |
miniCoopR |
Ceol et al., 2011
PMID: 21430779 |
||
| Recombinant DNA reagent | pcsDest2 |
Villefranc et al., 2007
PMID: 17948311 |
||
| Recombinant DNA reagent | p3E-polyA | Tol2Kit (Kwan et al., 2007) PMID: 17937395 |
||
| Recombinant DNA reagent | pME-eGFP | Tol2Kit (Kwan et al., 2007) PMID: 17937395 |
||
| Recombinant DNA reagent | pDestTol2CG2 (395) | Tol2Kit (Kwan et al., 2007) PMID: 17937395 |
||
| Recombinant DNA reagent | pDestTol2pA2 (394) | Tol2Kit (Kwan et al., 2007) PMID: 17937395 |
||
| Recombinant DNA reagent | miniCoopR-mitfa:dnBMPR:pA | This paper | miniCoopR vector expressing dominant negative BMP receptor; generated by multisite Gateway | |
| Recombinant DNA reagent | miniCoopR-mitfa:eGFP:pA | This paper | miniCoopR vector expressing control eGFP; generated by multisite Gateway | |
| Recombinant DNA reagent | miniCoopR-mitfa:SMAD1-DVD:pA | This paper | miniCoopR vector expressing constitutively active SMAD1; generated by multisite Gateway | |
| Recombinant DNA reagent | 395-mitfa:gdf6a:pA | This paper | pDEST vector expressing gdf6a under mitfa promoter; generated by multisite Gateway | |
| Recombinant DNA reagent | 395-mitfa:Cre-ERT2:pA | This paper | pDEST vector expression CreERT2 under mitfa promoter; generated by multisite Gateway | |
| Recombinant DNA reagent | pcsDest2-gdf6a | This paper | pDEST vector used to generate gdf6a probes; generated by Gateway reaction |
|
| Recombinant DNA reagent | pcsDest2-gdf6b | This paper | pDEST vector used to generate gdf6b probes; generated by Gateway reaction | |
| Sequence-based reagent | qPCR and Genotyping Primers | See Supplementary file 1 for primer sequences | ||
| Commercial assay or kit | LR Clonase 2+ Kit | Thermo-Fisher | #12538120 | |
| Commercial assay or kit | LR Clonase Kit | Thermo-Fisher | #11791043 | |
| Commercial assay or kit | Golden Gate TALEN and TAL Effector Kit 2.0 | Addgene | #1000000024 | |
| Commercial assay or kit | mMessage mMachine Kit | Ambion | #AM1340 | |
| Commercial assay or kit | DIG RNA Labeling Kit | Roche | #11175025910 | |
| Commercial assay or kit | SuperScript III First Strand Synthesis | Thermo-Fisher | #18080051 | |
| Commercial assay or kit | SYBR Green Master Mix | Applied Biosystems | 4344463 | |
| Commercial assay or kit | Click-it EdU Cell Proliferation Kit for Imaging, AF 555 | Invitrogen | C10338 | |
| Chemical compound, drug | DMH1 | Sigma Aldrich | #D8946 | |
| Chemical compound, drug | Hydroxytamoxifen (4-OHT) | Sigma Aldrich | #H7904 | |
| Chemical compound, drug | Epinephrine | Acros Organics | #430140250 | |
| Chemical compound, drug | DAPI | Life Technologies | #D1306 | |
| Chemical compound, drug | Hoechst-33342 | Life Technologies | #H3570 | |
| Chemical compound, drug | Pronase | Sigma Aldrich | #10165921001 | |
| Chemical compound, drug | Trizol | Ambion | #15596026 | |
| Software, algorithm | Microsoft Excel | Microsoft Excel (Office 2016), www.microsoft.com | ||
| Software, algorithm | Graphpad Prism 7 | GraphPad Prism seven for Windows, GraphPad Software, www.graphpad.com | ||
| Software, algorithm | Image J | (Schindelin et al., 2012) | ||
| Software, algorithm | Leica LAS X | Leica LAS X for Windows, Leica Microsystems, www.leica-microsystems.com | ||
| Software, algorithm | FlowJo | Flow Jo v10 for Windows, Beckton, Dickinson and Company, www.flowjo.com |