Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (M. musculus) | Neurod6Cre | PMID: 17146780 | RRID: MGI:4429427 | Dr Klaus Nave (MPI-EM) |
| Genetic reagent (M. musculus) | Emx1-CreERT2 | PMID: 16388308
Jackson laboratory |
Stock: 027784 RRID: IMSR_JAX:027784 |
|
| Genetic reagent (M. musculus) | Tbr2CreERT2 | PMID: 23897762 | RRID:MGI:5499789 | Dr Sebastian J Arnold (University of Freiburg) |
| Genetic reagent (M. musculus) | RCL-Gfp, RCE | PMID: 19363146
Jackson laboratory |
Stock: 32037 RRID: MGI:4420759 |
|
| Genetic reagent (M. musculus) | MADMTG | PMID: 21092859
Jackson laboratory |
Stock: 013751 RRID:IMSR_JAX:013751 |
|
| Genetic reagent (M. musculus) | MADMGT | PMID: 21092859
Jackson laboratory |
Stock: 013749 RRID:IMSR_JAX:013749 |
|
| Genetic reagent (M. musculus) | RCL-Fucci2a | PMID: 25486356 | RRID:IMSR_HAR:6899 | Dr Richard Mort (Lancaster University) |
| Cell line (Homo sapiens) | HEK293FT | Invitrogen | Cat: R700-07 RRID: CVCL_6911 |
|
| Transfected construct (M. musculus) | rv::dio-eGFP | PMID: 23933753
Addgene |
ID: 87662 | |
| Transfected construct (M. musculus) | rv::dio-mCherry | PMID: 23933753
Addgene |
ID: 87664 | |
| Antibody | Anti-GFP (chicken polyclonal) | Aves Lab | Cat: GFP-1020 RRID: AB_10000240 |
IF(1:2000) |
| Antibody | Anti-DsRed (rabbit polyclonal) | Clonetech | Cat: 632496 RRID: AB_10013483 |
IF(1:500) |
| Antibody | Anti-mCherry (goat polyclonal) | Antibodies online | Cat: ABIN1440057 RRID: AB_11208222 |
IF(1:500) |
| Antibody | Anti-Ctip2 (rat monoclonal) | Abcam | Cat: AB18465 RRID: AB_2064130 |
IF(1:500) |
| Antibody | Anti-Satb2 (mouse monoclonal) | Abcam | Cat: AB51502 RRID: AB_882455 |
IF(1:500) |
| Antibody | Anti-Satb2 (rabbit polyclonal) | Abcam | Cat: AB34735 RRID: AB_2301417 |
IF(1:1000) |
| Antibody | Anti-Tle4 (goat polyclonal) | Gift from Dr Stefano Stifani (McGill University) | IF(1:200) | |
| Antibody | Anti-chicken IgY-Alexa fluor 488 (goat polyclonal) | TermoFisher | Cat: A-11039 RRID: AB_2534096 |
IF(1:400) |
| Antibody | Anti-mouse IgG-Alexa fluor 647 (goat polyclonal) | TermoFisher | Cat: A-21240 RRID: AB_2535809 |
IF(1:400) |
| Antibody | Anti-mouse IgG-biotinilated (Horse polyclonal) | Vector Labs | Cat: BA2000 RRID: AB_2313581 |
IF(1:400) |
| Antibody | Anti-rat IgG-Alexa fluor 555 (goat polyclonal) | TermoFisher | Cat: A-21434 RRID: AB_2535855 |
IF(1:400) |
| Antibody | Anti-goat IgG-Alexa fluor 555 (donkey polyclonal) | TermoFisher | Cat: A-21432 RRID: AB_2535853 |
IF(1:400) |
| Antibody | Anti-rabbit IgG-Alexa fluor 488 (donkey polyclonal) | TermoFisher | Cat: A-21432 RRID: AB_2535853 |
IF(1:400) |
| Chemical compound, drug | Tamoxifen | Sigma-Aldrich | Cat: 85256 | |
| Software, algorithm | Prism 8 | GraphPad | RRID: SCR_002798 | |
| Software, algorithm | MATLAB | Mathworks | RRID: SCR_001622 | |
| Software, algorithm | RStudio | RStudio | RRID: SCR_000432 | |
| Software, algorithm | Imaris 8 | Bitplane | RRID: SCR_007370 |