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. 2020 Jan 17;6(3):eaax8582. doi: 10.1126/sciadv.aax8582

Fig. 3. Genetic engineering enables controlled composite response to specific cues.

Fig. 3

(A)Deletion of the HIS3 gene results in failure to proliferate in medium lacking histidine. (B) Schematic of a UV-patterned living composite with growth triggered by the amino acid l-histidine. (C) UV-patterned living composites with auxotrophic yeast do not substantially change in shape in medium lacking l-histidine. Shape change into a helical structure after incubation in medium containing l-histidine. Scale bar, 10 mm. (D) Volume change over time for auxotrophic living composites before and after l-histidine exposure. (E) Volume change over time for auxotrophic living composites incubated in medium lacking histidine, with d-histidine, or with l-histidine. (F) Schematic of a living microfluidic device where the composites forming the channels indicated in green contain living auxotrophic yeast. (G) Fluorescence image of fluid traversing the microfluidic device before exposure to medium (top left). Scale bar, 10 mm. Fluorescence image of fluid traversing the microfluidic device after medium containing l-histidine flows for 48 hours through the channels. Topography of a living channel before and after (color scale, 0 to 0.3 mm) growth (bottom). Scale bar, 1 mm. Each data point represents the mean (n = 3), and error bars represent SD. Trend lines are only intended to guide the eye.