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. Author manuscript; available in PMC: 2020 Jan 17.
Published in final edited form as: Opt Express. 2017 Nov 2;25(23):28489–28500. doi: 10.1364/OE.25.028489

Fig. 1.

Fig. 1

Optical layout for our simplified dual wavelength setup. A wavelength division multiplexer (WDM) is used to couple the two illumination wavelengths into a single fiber, which is directed onto a free-space coupler (FSC). An objective lens (OL) and tube lens (TL) produce an image of the sample at the microscope image plane, indicated as a vertical dashed line. The DualView apparatus (DV) images this plane with unit magnification onto a CMOS camera, spatially separating images created by the two illumination sources (see text).