Fig. 5.

Overexpression of TRPM7 protected against MPP⁺-induced cell death: a Protein lysates from control, MPP⁺(500 μM, 24 h), and MPP⁺ + TRPM7 overexpressed (transient expression for 48 h) SH-SY5Y cells were resolved on SDS gels and analyzed by western blotting. b Caspase 3 activity was evaluated under similar conditions (as labeled in the figure) in SH-SY5Y cells. The columns show the means ± SEM of 4 separate experiments performed in duplicate (*p < 0.05, **p < 0.01; one-way ANOVA, Tukey post hoc test). c MTT assays were evaluated under various conditions in SH-SY5Y cells. The columns show the means ± SD of 4 independent experiments (**p < 0.01; one-way ANOVA, Tukey post hoc test). d IV curves (acquired when currents reach its peak) of TRPM7 currents under various conditions and quantitation of current density at ± 100 mV (mean ± D of 6 experiments) are shown in (e). (**p < 0.01; one-way ANOVA)