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. 2020 Jan 17;10:544. doi: 10.1038/s41598-019-57318-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Δ9-THC decreases GLUT1 and GR in human BeWo trophoblast cells. Real-time qPCR of human BeWo cells treated with either vehicle, 15 µM Δ9-THC, or 15 µM 11-COOH-THC for 24 hours. Total RNA was extracted and reverse-transcribed to cDNA and normalized to GAPDH. All values were expressed as mean ± SEM (N = 6/group). Significant differences between treatment groups determined by 1-way ANOVA. Different letters represent means that are significantly different from one another according to Tukey’s post test (*P < 0.05, **P < 0.001).