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. 2020 Jan 17;10:537. doi: 10.1038/s41598-019-57287-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Examples of immunohistochemical staining of formalin-fixed, paraffin-embedded Capan-2 cancer cell pellets (A-B) and human pancreatic tissue microarrays (C–F) using novel mAb KU44.22B (15 μg/ml). Staining was performed as described in Methods section. (A) Negative control; (B) Staining of Capan-2 cells with mAb KU44.22B; (C) 2/3 + membrane, 2 + cytoplasm (C7); (D) 2 + membrane (B5); (E) 2 + membrane, 1 + cytoplasm (C3); (F) Normal pancreas tissue (F3); magnification 200×.